Polysorbate 80, also known as polyethylene glycol sorbitan monooleate, consists of sorbitol, ethylene oxide, and oleic acid. It has a molecular formula of C64H124O26. At room temperature, it is in the form of pale-yellow to amber oily liquid. It is an O/W emulsifier with a polysorbate 80 HLB value of 15.0. It is soluble in water, ethanol, ethyl acetate, and toluene. Polyethylene glycol sorbitan monooleate is a surfactant with numerous applications in food, pharmaceutical, and cosmetics; other than that, it can also use as an emulsifier for culture medium to reduce the surface tension of the medium, which enhances the growth of cells. Polyethylene sorbitan monooleate is also widely used in industrial productions such as use as a wetting agent and dispersant for photographic film making; as a stabilizer and foaming agent for polyurethane foaming plastic.