Humanized antibodies are derived from non-human species, modified to more closely resemble existing, natural human antibodies. Their applications have a great deal of potential in medicine and immunology due to the wide range of variability that humanized antibodies provide. Compared with heterologous antibodies, they also circumvent certain immune side effects to the human body. From chimeric antibodies to CDR-grafting to humanized antibodies, each major breakthrough has helped expand the field of antibody preparation and advance the cutting edge of antibody library technology .
The development of therapeutic antibodies has been tending toward the use of fully humanized antibodies, which are typically derived from antibody libraries. The development of antibody library technology makes it possible to more easily obtain antibodies in vitro.